CR decreases Mcl-1 expression. WT C57BL/6 syngenic mice were injected intravenously with Eµ-Myc lymphoma cells and fed ad libitum or under CR conditions (CR, 75% of normal dose; see “Methods”). (A) Average weight of the mice that were fed for 23 days ad libitum (Ctl, control) or under CR conditions (Ctl, n = 10; CR, n = 9). (B) Glycemia was measured after 9 days of dietary study (Ctl, n = 10; CR, n = 9). **P < .01. (C) Lymph nodes bearing lymphoma were harvested from 5 independent mice after 24 days under CR or ad libitum feeding (control) and lysates were prepared. Bcl-2 family member expression was analyzed by immunoblots. (D) Average quantification of Mcl-1, Puma, Bim, and Bim S levels compared with Erk2 levels (used as a loading control) from samples in (C) (n = 5 per group). Reduction of Mcl-1, 39% ± 10%. *P < .05. (E) Lymph nodes bearing lymphoma were harvested from 5 independent mice as in (C) and the AMPK/mTOR pathway was analyzed by immunoblots. ns, nonsignificant.