CR mimetics restore the sensitivity of primary Eµ-Myc cells to ABT-737, independently of p53. (A) The indicated MEF and Eµ-Myc lymphoma cells were treated with increased concentrations of ABT-737 for 20 hours. Cell death was determined by propidium iodide (PI) staining and analyzed by fluorescence-activated cell sorter (FACS). The insert shows Mcl-1 expression. (B) Eµ-Myc/WT or Eµ-Myc/p53^−/− cells were incubated with 2DG 100 µg/mL or LND 100 µM for 20 hours, and the level of Mcl-1 was analyzed by immunoblot. In (A) and (B), HSP60 was used as a loading control. Quantifications of Mcl-1 over HSP60 levels are indicated. (C-D) Indicated cells were treated with 1, 5, or 10 µM ABT-737 with or without 75 µg/mL 2DG (C) or 100 µM LND (D). The percentage of PI-positive dead cells was measured as in (A). The results are expressed as the mean ± standard deviation (SD) from 3 independent experiments. Each experiment was performed on cells from 3 independently derived lymphomas. ***P < .001.