Loss of Bim does not prevent the CR-mediated sensitization to ABT-737–induced cell death in primary Eµ-Myc cells. (A) WT or Bak/Bak double-knockout MEF cells were incubated with indicated amount of ABT-737 for 20 hours with or without 500 µg/mL 2DG with or without 20 µM qVD-OPH, and cell death was measured as in Figure 3A. (B) Indicated cells were treated with 20 µM ABT-737 ± 500 µg/mL 2DG ± 20 µM qVD-OPH. After 4 days, the number of clones was counted. (C) Schematic representation of the interaction between the pro- and antiapoptotic members of the Bcl-2 family. (D) The indicated MEF and Eµ-Myc cells were incubated with 0.5, 1, 5, or 10 µM ABT-737. The percentage of PI-positive cells was determined by FACS. Insert: immunoblot of Mcl-1 expression. (E) Eµ-Myc/Bim^−/− cells were incubated with 2DG 100 µg/mL or LND 100 µM for 20 hours, and the levels of Mcl-1 were analyzed by immunoblot. HSP60 was used as a loading control. Quantifications of Mcl-1 over HSP60 levels are indicated. (F-G). Eµ-Myc/Bim^−/− cells were treated with the indicated doses of ABT-737 in the presence or not of 2DG 75 µg/mL (F) or LND 100 µM (G) for 20 hours. Cell death was measured as in Figure 3A. Results shown are mean ± SD from 3 independent experiments. Each experiment was performed on cells from 2 independently derived lymphomas. ***P < .001.