Figure 6
Figure 6. There is no compensatory effect of Bim, Puma, or Noxa on the CR mimetics–mediated sensitization to ABT-737–induced death. (A) The indicated cells were treated for 20 hours with increasing doses of ABT-737. Cell death (percentage of PI-positive cells) was determined by FACS. The insert shows Mcl-1 expression in the indicated cell types. (B) The indicated cells were incubated with 2DG 100 µg/mL or LND 100 µM for 20 hours, and the levels of Mcl-1 were analyzed by immunoblot. HSP60 was used as a loading control. Quantifications of Mcl-1 over HSP60 levels are indicated. (C-D) The indicated cells were treated for 20 hours with 1, 5, or 10 µM ABT-737 with or without 2DG 100 µg/mL or LND 100 µM. Dead cells were counted as in Figure 3A. The results represent the mean ± SD of 3 independent experiments. Each experiment was performed on cells from 2 independently derived lymphomas for each genotype. ***P < .001.

There is no compensatory effect of Bim, Puma, or Noxa on the CR mimetics–mediated sensitization to ABT-737–induced death. (A) The indicated cells were treated for 20 hours with increasing doses of ABT-737. Cell death (percentage of PI-positive cells) was determined by FACS. The insert shows Mcl-1 expression in the indicated cell types. (B) The indicated cells were incubated with 2DG 100 µg/mL or LND 100 µM for 20 hours, and the levels of Mcl-1 were analyzed by immunoblot. HSP60 was used as a loading control. Quantifications of Mcl-1 over HSP60 levels are indicated. (C-D) The indicated cells were treated for 20 hours with 1, 5, or 10 µM ABT-737 with or without 2DG 100 µg/mL or LND 100 µM. Dead cells were counted as in Figure 3A. The results represent the mean ± SD of 3 independent experiments. Each experiment was performed on cells from 2 independently derived lymphomas for each genotype. ***P < .001.

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