Figure 2
IL-1β overproduction from FANCC-deficient macrophages is p38 dependent. T-shNT and T-shFC cells were plated at a concentration of 106 per mL, pretreated with BIRB 796 (50 nM) for 6 hours, and stimulated with R848 (30 μM) for 24 hours. Secreted IL-1β (A) and TNF-α (B) were measured in the conditioned media by ELISA. One experiment with 3 technical replicates for each condition is shown. (C) Western blotting of indicated proteins from whole cell extracts of the same cells was performed, and molecular weight markers are indicated (casp-1 indicates caspase-1). Band densitometry was performed using ImageJ software. Phosphorylated MK2 band density was normalized to total MK2 band density. P values were calculated using a paired Student t test.
