Teffincrease FA metabolism during GVHD but not following cellular immunization. (A) FA transport was measured in OT-I cells from naïve donors or in OT-I cells 7 days after either CAG.OVA dendritic cell immunization (Imm) or transplantation into irradiated CAG.OVA mice (GVHD), n = 4 mice/bar. (B) OT-I T cell lysates (following flow-sorting) were probed for PGC-1α levels by western blot as in Figure 2E. (C) B6 Ly5.2 (CD45.1+) splenocytes were labeled with CellTrace violet and transferred to nonirradiated B6D2F1 animals (GVHD) as described in “Methods.” On day 7, donor T cells were recovered and measured for BoDipyC1-C12 uptake. B6 Ly5.2 T cells transferred to nonirradiated C57Bl/6 animals served as controls (Syn). Bar graphs represent 4 to 5 mice/group. ***P < .007.
