Blockade of FA oxidation preferentially targets well-divided, allogeneic T cells. (A) T cells were purified by magnetic separation and cultured ex vivo with B6D2F1 splenocytes with or without etomoxir as detailed in Methods. (B) Mice received a single dose of etomoxir on day 7 and total donor T-cell numbers were measured 16 hours later. (C) AnnexinV staining of donor T cells 16 hours after etomoxir treatment. (D) C57Bl/6 recipient mice were transplanted with BM and T cells from C3H.SW donor mice as detailed in “Methods.” Beginning on day +5, recipient mice received either PBS (black circles) or etomoxir (gray triangles) every other day for a total of 2 weeks. Clinical scores, as described previously,17 were measured on day 29, 10 days after the discontinuation of etomoxir. The mean clinical score is represented by a solid black line. *P = .02, **P < .01.
