Improved ratio of CD4⁺ to CD8⁺ T cells in mC46 macaques. (A) The ratio of CD4⁺CD3⁺ T cells over CD8⁺CD3⁺ T cells was determined by FACS analysis throughout the course of these studies. Results shown are for both mC46-expressing macaques (circles and triangles) and both control macaques (squares and diamonds). (B-C) The relative percentage of CD4⁺ T cells and CD8⁺ T cells in mixed-cell populations isolated from (B) GI and (C) LN samples was determined by FACS analysis. Results for second set are shown before SHIV challenge and after 6 months; averages from both sets are shown in supplemental Figure 2 in addition to time points taken at days 7 and 21 post-SHIV challenge.