TKO-BLT mice have significantly better human peripheral blood reconstitution than DKO-BLT mice. Peripheral blood was collected and analyzed by flow cytometry at the indicated time points to assess the effect of CD47 expression on (A) overall levels of human CD45⁺ cell reconstitution and (B) the frequencies of the major human leukocyte subsets in the blood. Representative batches of ≥9 mice are shown at each time point. Boxes indicate median and interquartile range. Whiskers are minimum and maximum values. Unpaired 2-tailed t tests were performed between groups at each time point. ****P < .0001; ***P < .001; **P < .01; *P < .05.