TKO-BLT mice mount specific humoral and cellular immune responses to HIV infection. (A) Plasma collected at 8 wpi from 6 intraperitoneally infected mice injected with 3000, 7000, or 20 000 TCIU of HIV_JR-CSF or 2 uninfected vehicle control injected mice was assayed by ELISA for combined IgM, IgA, or IgG antibodies or only IgG antibodies specific for HIV_JR-CSF gp120 protein. Each line represents data from a single mouse. (B) At 7 wpi, 3 pooled spleens from HIV⁺ mice and 3 control HIV⁻ spleens were depleted of murine leukocytes prior to ELISpot assay to determine specific cellular responses to pools of 8 overlapping HIV peptides that covered the HIV_JR-CSF proteome. Positive responses (denoted by an asterisk) had >50 spots/10⁶ splenocytes and were twofold over vehicle-only (dimethylsulfoxide) controls.