Induction of platelet activation in L5-injected mice. L5, L1 (5 mg/kg each), or PBS (Ctl) was injected into the tail vein of adult male C57BL/6 mice twice a week for 6 weeks (n = 3 for each treatment group). (A) Tail bleeding time was significantly shortened in L5-injected mice. **P < .01, determined by using 1-way analysis of variance with the Bonferroni post hoc test. When the same experiment was performed in LOX-1 knockout mice, the effect of L5 on tail bleeding time was attenuated. Whole blood drawn from mice was anticoagulated with heparin. Platelets were collected, stained with P-selectin and GPIIb/IIIa monoclonal antibodies, and subjected to flow cytometry. (B) P-selectin and (C) GPIIb/IIIa activation was significantly higher in L5-injected mice than in PBS-injected mice.