American Society of Hematology

Figure 4

$Figure 4. DNA hypomethylation around the minigene locus of HSCs. (A-B) MeDIP assay of the G1HE, dbG, and IE promoter regions in the sorted c-Kit−positive progenitor cells and the Ter119-positive erythroblasts from the G1B-GFP line 392 and the MG-GFP line 20 transgenic mice. The data are normalized to the DNA methylation level at the Actb locus. A representative dataset from these experiments, which were repeated 3 times, is shown. The statistical significance of differences is indicated (**P < .01; *P < .05; n.s., not significant; Student unpaired t test). (C-D). Bisulfite sequencing analysis of the G1TAR, G1HE, dbG, and IE promoter regions in the sorted LSK fraction of the G1B-GFP line 392 and the MG-GFP line 20 transgenic mice, respectively. The numbers indicate each CpG motif in the each regulatory region. Both methylated (filled circles) and unmethylated (open circles) CpG motifs are depicted. The DNA methylation ratio of the each locus is denoted by the percentage.$

DNA hypomethylation around the minigene locus of HSCs. (A-B) MeDIP assay of the G1HE, dbG, and IE promoter regions in the sorted c-Kit−positive progenitor cells and the Ter119-positive erythroblasts from the G1B-GFP line 392 and the MG-GFP line 20 transgenic mice. The data are normalized to the DNA methylation level at the Actb locus. A representative dataset from these experiments, which were repeated 3 times, is shown. The statistical significance of differences is indicated (**P < .01; *P < .05; n.s., not significant; Student unpaired t test). (C-D). Bisulfite sequencing analysis of the G1TAR, G1HE, dbG, and IE promoter regions in the sorted LSK fraction of the G1B-GFP line 392 and the MG-GFP line 20 transgenic mice, respectively. The numbers indicate each CpG motif in the each regulatory region. Both methylated (filled circles) and unmethylated (open circles) CpG motifs are depicted. The DNA methylation ratio of the each locus is denoted by the percentage.

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