Neutrophil depletion inhibits immunization-induced lymphangiogenesis in footpads in WT mice. (A) Footpad sections were immunostained for Ly6G and LYVE-1 to examine neutrophil accumulation and lymphatic vessel density after CFA/ KLH immunization. (B-C) Footpad skin whole mounts from neutrophil-depleted and control rat IgG-treated WT mice were examined for lymphatic vessel network (B) and density (C) at 14 days postimmunization. (D) Percentage of BrdU⁺ proliferating LECs in inflamed footpads of mice that received NIMP-R14 mAb or control rat IgG treatment. (E) Accumulation of nongranulocytic myeloid cells in inflamed footpad sections from NIMP-R14-treated mice. (F) Footpad swelling in NIMP-R14 mAb- and control rat IgG-treated mice. Data from panel C are pooled from 2 independent experiments with 2 to 3 mice per group in each experiment (n = 4-6). Data from panel D consist of 5 to 6 mice per group (n = 5-6). Bars represent mean ± SD. **P < .01. Images are representative of 3 independent experiments (n = 3) for panel A and 6 independent experiments (n = 6) for panels B, E, and F. Scale bars in panels A, B, and E represent 100, 75, and 200 μm, respectively. Scale bar in panel F represents 2 mm.