Attenuating neutrophil accumulation inhibits lymphangiogenesis in chronic CHS. (A) Ear sections were immunostained for Ly6G and LYVE-1 to examine neutrophil infiltration and lymphatic vessels after DNCB sensitization. (B) Neutrophil accumulation and lymphatic vessels in DNCB-challenged ears from NIMP-R14- and control rat IgG-treated mice. (C) Density of lymphatic vessels in DNCB-challenged ears from NIMP-R14- and control rat IgG-treated mice. (D) Percentage of BrdU⁺ proliferating LECs in DNCB-challenged ears of mice that received NIMP-R14 or control rat IgG treatment. (E) Ear thickness of DNCB-challenged ears from NIMP-R14- and control rat IgG-treated mice. (F) Hematoxylin and eosin staining of control or challenged ears from NIMP-R14- and control rat IgG-treated mice. Data from panel D consist of 4 mice per group (n = 4). All other data shown are representative or pooled from 2 independent experiments with 3 mice per group in each experiment (n = 6). *P < .05; **P < .01. Bars represent mean ± SD. Images are representative of 3 independent experiments (n = 3) for panel A and 5 independent experiments (n = 5) for panels B and F. Scale bars in the right and left panels of panel A represent 100 and 75 μm, respectively. Scale bars in the right and left panels of panel B represent 75 and 50 μm, respectively. Scale bar in panel F represents 2 mm. Dotted lines denote boundary of ear sections.