Ezh2 deficiency does not affect the initial activation and proliferation of donor T cells during alloantigen-priming phase. (A-C) Donor CD44^lo T cells (ie, CD4⁺ + CD8⁺ T cells) derived from WT or T-KO B6 mice were labeled with CFSE and transplanted with TCD BM into lethally irradiated BALB/C recipients. Three days later, donor T cells were recovered from these BALB/C recipients for analysis. (A) Dot plots show the fraction of donor-derived H2^b+ CD4⁺ or CD8⁺ T cells, and graphs show the percentage and absolute number of donor T cells. (B) Histograms show the CFSE dilution in donor T cells activated in allogeneic recipients. Graphs show the percentage of donor-derived T cells at each division. (C) Cells were collected for flow cytometry analysis. Histograms show the expression of CD25, CD69, CD122, and CD44 in donor-derived T cells. (D) CD44^lo T cells derived from WT or T-KO B6 mice were stimulated by BALB/C BM-derived DCs. Three days later, cells were pulsed with BrdU for 2 hours to measure the incorporation of BrdU. Histograms and graphs show the percentage of T cells with BrdU incorporation. *P < .05, **P < .01. Error bars indicate mean ± SD. Data are representative of 2 independent experiments.