American Society of Hematology

Figure 6

$Figure 6. Ezh2-deficient T cells are specifically impaired for their differentiation into effector cells producing IFN-γ Donor T cells (CD4+ + CD8+ T cells) derived from WT or T-KO B6 mice were transplanted with B6 TCD BM into lethally irradiated BALB/C recipients. At indicated time points after transplantation, the cells were collected, counted, and analyzed using flow cytometry. (A) The percentage of donor T cells producing IFN-γ. (B) Graphs show cytokine production assessed by ELISA. (C) The fraction of donor T cells producing IL-4. (D) The percentage of donor T cells producing TNF-α, IL-17, and IL-2. (E) RT-PCR analysis of gene expression in donor CD4+ T cells isolated from the spleens of BMT recipients 7 days after transplantation. **P < .01. Error bars indicate mean ± SD. Data are representative of 2 independent experiments with 3 to 5 mice per group.$

Ezh2-deficient T cells are specifically impaired for their differentiation into effector cells producing IFN-γ Donor T cells (CD4⁺ + CD8⁺ T cells) derived from WT or T-KO B6 mice were transplanted with B6 TCD BM into lethally irradiated BALB/C recipients. At indicated time points after transplantation, the cells were collected, counted, and analyzed using flow cytometry. (A) The percentage of donor T cells producing IFN-γ. (B) Graphs show cytokine production assessed by ELISA. (C) The fraction of donor T cells producing IL-4. (D) The percentage of donor T cells producing TNF-α, IL-17, and IL-2. (E) RT-PCR analysis of gene expression in donor CD4⁺ T cells isolated from the spleens of BMT recipients 7 days after transplantation. **P < .01. Error bars indicate mean ± SD. Data are representative of 2 independent experiments with 3 to 5 mice per group.

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