Figure 1
Figure 1. Rasip1 knockout mice die in midgestation with vascular defects. Brightfield images of mouse embryos (A-G) with the indicated ages and Rasip1 genotypes, showing pericardial edema and multifocal hemorrhage in Rasip1−/− embryos at E9.5 and E10.5; Rasip1−/− embryos were also significantly smaller than control at E10.5. Immunofluorescence staining of CD31 plus CD105 (red) and DAPI (blue) in whole-mount embryos (H-K) or transverse sections of DA (L-O) with the indicated ages and genotypes revealed irregular DA in Rasip −/− embryos. RBC autofluorescence (green) in panels J-O showed that erythrocytes were present in dilated vessels or in extravascular space in Rasip −/− embryos. Arrows indicate DA. DAPI, 4,6 diamidino-2-phenylindole; so, somite; ss, somite stage. Panels H-I, ventral view, rostral to the left; panels J-K, lateral view. Scale bars: (A-B,E-G) 0.5 mm; (C-D) 1 mm; (H-I) 50 μm; (J-K) 100 μm; (L-O) 10 μm.

Rasip1 knockout mice die in midgestation with vascular defects. Brightfield images of mouse embryos (A-G) with the indicated ages and Rasip1 genotypes, showing pericardial edema and multifocal hemorrhage in Rasip1−/− embryos at E9.5 and E10.5; Rasip1−/− embryos were also significantly smaller than control at E10.5. Immunofluorescence staining of CD31 plus CD105 (red) and DAPI (blue) in whole-mount embryos (H-K) or transverse sections of DA (L-O) with the indicated ages and genotypes revealed irregular DA in Rasip−/− embryos. RBC autofluorescence (green) in panels J-O showed that erythrocytes were present in dilated vessels or in extravascular space in Rasip−/− embryos. Arrows indicate DA. DAPI, 4,6 diamidino-2-phenylindole; so, somite; ss, somite stage. Panels H-I, ventral view, rostral to the left; panels J-K, lateral view. Scale bars: (A-B,E-G) 0.5 mm; (C-D) 1 mm; (H-I) 50 μm; (J-K) 100 μm; (L-O) 10 μm.

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