DOCK2 regulates the lytic synapse formation through Rac activation. (A) NKG2D-mediated Rac activation was compared between WT and DOCK2^−/− NK cells. (B) NKG2D-mediated phosphorylations of Vav and PLCγ2 were compared between WT and DOCK2^−/− NK cells. (C) NKG2D-mediated phosphorylations of Erk, JNK, SLP76, and Akt were compared between WT and DOCK2^−/− NK cells. (D) After adenoviral transfer of GFP-tagged WT DOCK2, DOCK2^V1538A, or GFP alone, WT and DOCK2^−/− NK cells were mixed with YAC-1 cells for 5 minutes to analyze localization of perforin and F-actin in the conjugates. Scale bar, 5 μm. Data are expressed as the percentages of the conjugates with polarized accumulation of perforin or F-actin (the mean ± SD) of 3 independent experiments. In each experiment, more than 30 cells were analyzed per group. *P < .05; **P < .01.