American Society of Hematology

Figure 6

$Figure 6. AhR repression promotes megakaryocyte specification. (A) Schematic representation of a pHAGE2 lentiviral reporter construct containing the AhRR and ZsGreen under the control of the constitutive promoter Ef1α (pHAGE2-Ef1α-AhRR-IRES-zsGreen). (B) Phase-contrast and fluorescent images of D30 HPs after infection with a constitutively active ZsGreen control virus or pHAGE2-Ef1α-AhRR-IRES-zsGreen. Large cells (megakaryocytes) are noted in the populations infected with the AhRR. (C) Graphical representation of the percentage of ZsGreen+ cells that express CD235 (erythroid lineage) or CD41 (megakaryocyte lineage) after mock or AhRR infection. Data are presented as means of 3 independent experiments ± SD: *P < .005. (D) FACS analysis of AhRR-ZsGreen negative vs positive fractions for hallmark megakaryocyte markers CD41a/CD42b.$

AhR repression promotes megakaryocyte specification. (A) Schematic representation of a pHAGE2 lentiviral reporter construct containing the AhRR and ZsGreen under the control of the constitutive promoter Ef1α (pHAGE2-Ef1α-AhRR-IRES-zsGreen). (B) Phase-contrast and fluorescent images of D30 HPs after infection with a constitutively active ZsGreen control virus or pHAGE2-Ef1α-AhRR-IRES-zsGreen. Large cells (megakaryocytes) are noted in the populations infected with the AhRR. (C) Graphical representation of the percentage of ZsGreen⁺ cells that express CD235 (erythroid lineage) or CD41 (megakaryocyte lineage) after mock or AhRR infection. Data are presented as means of 3 independent experiments ± SD: *P < .005. (D) FACS analysis of AhRR-ZsGreen negative vs positive fractions for hallmark megakaryocyte markers CD41a/CD42b.

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