Distribution and phenotype of splenic T-cell subsets in Rag1-mutant mice. (A) Representative example of CD4⁺ and CD8⁺ T cells (top) and of CD4⁻CD8⁻ cells expressing the γδ form of the TCR (bottom) among splenic CD3⁺ cells. Percentages (among CD3⁺ cells) (B) and absolute count of CD4⁺ and CD8⁺ cells (C). (C) Gating strategy for naïve (CD62L⁺CD44⁻), central memory (CD44⁺CD62L⁺), and effector memory T cells (CD44⁺CD62L⁻) among CD4⁺ (top) and CD8⁺ (bottom) T cells. (D) Distribution of naïve, central memory, and effector memory T cells among total splenic CD4⁺ (top) and CD8⁺ (bottom) cells (n = 6 per group). Error bars represent standard error of the mean. (B,D) One-way analysis of variance: *P ≤ .05, **P ≤ .01, ***P ≤ .001, ****P ≤ .0001.