Abnormalities of Trb repertoire in Rag1-mutant mice. (A) Percentage of productive and nonproductive reads of total unique Trb joins from WT and R972Q DN4 thymocytes (CD4⁻CD8⁻CD44⁻CD25⁻); WT, R972Q, and F971L CD4⁺CD8⁺ DP thymocytes; WT, R972Q, and F971L T_conv (CD4⁺EGFP⁻) cells; and WT, R972Q, and F971L T_reg (CD4⁺EGFP⁺) cells. (B) Utilization of V (left) and J (right) genes in Trb rearrangements in DN4 thymocytes (CD4⁻CD8⁻CD44⁻CD25⁻). Top shows WT mice; bottom shows R972Q mice. (C) Trb unique sequences in CD4⁺CD8⁺ DP thymocytes. Top shows WT mice; bottom shows R972Q mice. (D) Trb unique sequences in CD4⁺CD8⁺ DP thymocytes. Top shows WT mice; bottom shows F971L mice. (E) Trb unique sequences in splenic T_conv (CD4⁺EGFP⁻) cells from WT (top) and R972Q (bottom) mice. (F) Trb unique sequences in splenic T_conv (CD4⁺EGFP⁻) cells from WT (top) and F971L (bottom) mice. (G) Utilization of V (left) and J (right) genes in Trb unique sequences in splenic T_reg (CD4⁺EGFP⁺) cells from WT (top) and R972Q (bottom) mice. (H) Utilization of V (left) and J (right) genes in Trb unique sequences in splenic T_reg (CD4⁺EGFP⁺) cells from WT (top) and F971L (bottom) mice. χ² test: *P ≤ .05, ***P ≤ .001, ****P ≤ .0001.