Immunohistochemical analysis of proinflammatory markers in livers from chimeric mice. Immunostaining for (A) E-selectin, (B) VCAM-1, (C) ICAM-1, (D) TF, and (E) MCP-1 in livers from LPS-treated S1pr2^+/+ → S1pr2^+/+, S1pr2^+/+ → S1pr2^−/−, and S1pr2^−/− → S1pr2^+/+ mice 18 hours after LPS injection. (A-C) Note that immunoreactivity for adhesion molecules was markedly decreased in venules and liver sinusoids only in the S1pr2^+/+ → S1pr2^−/− mice compared with S1pr2^+/+ → S1pr2^+/+ mice. (D) TF immunoreactivity was also significantly lower in infiltrating leukocytes from the S1pr2^+/+ → S1pr2^−/− chimeras. (E) Significantly lower levels of MCP-1 were detected only in hepatocytes and leukocytes from S1pr2^+/+ → S1pr2^−/− mice compared with S1pr2^+/+ → S1pr2^+/+ mice. Scale bar, 50 μm. Representative fields from 3 to 5 mice are shown. Images were captured with the Axio Imager A1 microscope, using AxioCam MRc camera and the AxioVision 4.8 program (Carl Zeiss Inc.) (original magnification ×40). h, hepatocyte; le, leukocyte; s, sinusoid; v, venule.