Knockdown of FOXO3 reduces γ-globin expression and protein levels relative to β-globin, and overexpression of FOXO3 increases γ-globin expression and protein levels relative to β-globin. (A) An 80% knockdown of FOXO3 is achieved. (B) Effects of FOXO3 knockdown on γ-globin and β-globin, as measured by RT-qPCR (3 independent assays). (C) Western blot depicting FOXO3 knockdown and its effect on γ-globin and β-globin protein levels. (D) Western blot depicting effects of FOXO3 overexpression on γ-globin and β-globin. (E) Quantification of western blot (3 independent assays). Error bar represents SD. ***P < .0001, **P < .005, *P < .05. NS, not significant.