Myc stimulates the BCR response in a miR-17∼92–dependent manner. (A-D) Western blotting was performed for P-SYK, total-SYK, P-BLNK, total-BLNK, and GAPDH. (A) P493-6 cells were grown in the presence (MYC^LOW) of 1μM doxycycline and treated with control mimic or miR-17∼92 mimic mix for 48 hours prior to addition of 10 μg/mL soluble anti-IgM for 15 minutes. (B) P493-6 cells were grown in the presence (MYC^LOW) of 1μM doxycycline and treated with control mimic or miR-17∼92 mimic mix for 48 hours prior to treatment with immobilized anti-IgM (imm-α-IgM) or immobilized isotype control (imm-isotype-CTRL) for 6 hours. (C) P493-6 cells were grown in the absence (MYC^HIGH) of 1μM doxycycline and treated with control short-hairpin inhibitor or miR-17∼92 short-hairpin inhibitor mix for 48 hours prior to addition of 10 μg/mL soluble anti-IgM for 15 minutes. (D) P493-6 cells were grown in the absence (MYC^HIGH) of 1μM doxycycline and treated with control short-hairpin inhibitor or miR-17∼92 short-hairpin inhibitor mix for 48 hours prior to treatment with immobilized anti-IgM (imm-α-IgM) or immobilized isotype control (imm-isotype-CTRL) for 6 hours.