Clonal dynamics in mice transplanted with barcoded cells. (A) Barcode composition of granulocytes, T- and B lymphocytes in one of the mice transplanted with ∼26 barcoded HSCs. Different colors reflect different barcodes. This and other mice shown here originate from a cohort of mice transplanted with nonsorted transduced LSK48⁻150⁺ cells (these mice are identified with light gray circles as shown in Figure 2). (B) Pearson correlations between barcode compositions of 3 cell types at 4 time points from 6 mice transplanted with ∼26 HSCs. Note good correlations between samples collected from 12 to 24 weeks within each of the cell types. Mouse 1 corresponds to the data shown in (A). (C) The same analysis as in panel (B) was performed for 3 mice transplanted with 100 barcoded cells. At these doses, Poisson distribution predicts that more than two-thirds of the positive animals are transplanted with a single HSC.