Figure 2
![Figure 2. TEL-AML1 induces phosphorylation of STAT3. (A) Western blot analysis of p-S727 and p-Y705 STAT3 and total STAT3 in mouse c-kit+Ter119- fetal liver HPC, 72 hours after transduction with murine stem cell virus TEL-AML1 expressing (T/A) or empty (MSCV CD2) retroviral vectors. Numbers represent densitometric quantitation of phospho-STAT3 bands normalized to total STAT3. (B) Flow cytometric analysis of p-Y705 STAT3 in Reh 6 days after transduction with control scramble (mean fluorescence intensity [MFI] = 21.04) or shTA shRNA (MFI = 12.01) shRNA. Isotype control staining (MFI = 10.23) is also shown. All data are representative of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/122/4/10.1182_blood-2012-11-465252/4/542f2.jpeg?Expires=1735502163&Signature=kokMuCCjVyZiOYhxf-VCiKKCbFP8WIclwNJpqVpoMKyLy550OcqO~nauYjjfk8nPksyxrZNTxpYfxIcKJkZnOmoDcu7EFnMBNZg~xSD0O6Leyvev3-F~cgQrL1Y8Tn6pWeZEwIGrHyHERoYyP~x~ZPok6ogx9vHCkmsaK-0smFACYErA3ZBm81c2vtz0nHMoE8LV2Bu-ia1w5Fwh-go8wzctoevSJmb6LFo5hfGk~jVlpA9tXCWmLT4F18lexL3HoT0PE~coXMkK35zmj9uKFopEEcp7mJSJ5z9BrPGaDxCUUbpgFX2yqz4J-UV16y~KdQ2RRAo4SLpKi0DCJGoNAA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TEL-AML1 induces phosphorylation of STAT3. (A) Western blot analysis of p-S727 and p-Y705 STAT3 and total STAT3 in mouse c-kit+Ter119- fetal liver HPC, 72 hours after transduction with murine stem cell virus TEL-AML1 expressing (T/A) or empty (MSCV CD2) retroviral vectors. Numbers represent densitometric quantitation of phospho-STAT3 bands normalized to total STAT3. (B) Flow cytometric analysis of p-Y705 STAT3 in Reh 6 days after transduction with control scramble (mean fluorescence intensity [MFI] = 21.04) or shTA shRNA (MFI = 12.01) shRNA. Isotype control staining (MFI = 10.23) is also shown. All data are representative of 3 independent experiments.
