STAT3 is necessary for colony formation by TEL-AML1⁺ cells. (A-C) Colony formation in methylcellulose by Reh cells 6 days after transduction with control scramble or shTA (A), shSTAT3 (B), or shMYC (C) shRNA. Plots show the number of colonies formed relative to control cultures, columns representing mean ± standard deviation values of triplicate cultures. Data are representative of 3 independent experiments. **P < .01, ***P < .005 compared with control (Student unpaired t test). CFU, colony-forming unit.