Abnormal terminal differentiation of megakaryocytes in Mx1-C1 mice. (A) Ploidy analysis of femur bone marrow CD41⁺ megakaryocytes form WT and Mx1-C1 mice. (B) Bar graph of megakaryocyte ploidy analysis. Data represent the mean ± standard deviation (n = 3). (C) Megakaryocytes were stained for CD41 (green), vWF (magenta), and 4,6 diamidino-2-phenylindole (DAPI) (blue). Both WT and Mx1-C1 megakaryocytes exhibit finely granular cytoplasmic staining by anti-vWF Ab. Scale bars, 10 μm. (D) Proplatelet-forming megakaryocytes obtained from WT and Mx1-C1 mice were cultured on fibrinogen-coated glass slides for 14 hours. These proplatelet-forming megakaryocytes were stained for β-tubulin. Scale bars, 50 μm. (E) The percentage of proplatelet-forming megakaryocytes in CD41⁺ cells was analyzed by fluorescence microscopy. Primary megakaryocytes were plated on fibrinogen-coated (FNG) glass slides or plated in the absence of adhesive proteins (None). Values represent the means ± SEM. *P < .01.