Expression of megakaryocytic-/platelet-specific glycoproteins in Mx1-C1 mice. (A) HPA lectin staining of WT and Mx1-C1 megakaryocytes. Merged image of anti-CD41 (green), HPA lectin (magenta), and DAPI (blue). Scale bars, 10 μm. (B) Quantitative analysis of transcription of megakaryocytic-/platelet-specific glycoproteins in bone marrow using RT-PCR. The expression of each transcript was normalized to that of the gapdh transcript. Data were obtained from triplicate experiments and presented as means ± SEM. *P < .01. (C) Flow cytometric analysis of the expression of CD41 and CD42 (GPIbα) in PRP prepared from WT and Mx1-C1 peripheral blood. The expression of CD42 was represented in a fraction of CD41⁺ cells within PRP. (D) Flow cytometric analysis of the expression of CD42 (GPIbα) in CD41-enriched cells prepared from WT and Mx1-C1 bone marrow cells. (E) Western blot analysis of platelet or enriched megakaryocyte extracts with anti-GPIbα and anti–β-actin Abs as internal controls.