Increased thrombus formation in ACC DKI mice is prevented by P2Y12 inhibition. (A-B) Whole-blood flow perfusion experiments were performed with collagen type I as platelet-adhesive substrate. Where indicated, autocrine effects were blocked by preincubation with 20 µM ticagrelor (TICA) or vehicle (dimethyl sulfoxide) for 10 minutes. Microscopic images were analyzed for indicated parameters, and per parameter normalized on scale from 0 to 10.²⁴  (A) Heat map of normalized parameters. (B) Subtraction heat map of normalized differences compared with WT mice, filtered for changes with P ≤ .05.