Lack of ACC phosphorylation does not affect oxidative metabolism. (A-F) Oxygen consumption rate (OCR) was measured in washed platelets pretreated or not with Etomoxir (25 μM) (E-F) for 1 hour before bioenergetic measurements. OCR was assessed under basal conditions, after injection of media alone or 1 U/mL thrombin (A,B) or 25 μg/mL collagen (C-D) or followed by treatment with 1 μM oligomycin (O), 0.1 μM carbonyl cyanide p-trifluoromethoxyphenylhydrazone (F), and a mix of 1 μM antimycin A (aA) and 1 μM rotenone (R). (A,C,E) Representative OCR profiles with thrombin (A) and collagen stimulation (C) or Etomoxir treatment (E). (B,D) Mitochondrial function was assessed by calculating basal, thrombin-induced (B) or collagen-induced (D), ATP-linked, maximal, nonmitochondrial, reserve capacity and proton leak OCR. In addition, bioenergetics measurements were assessed in Etomoxir-treated platelets (F). The results are expressed as means ± SEM (at least n = 3). *P ≤ .05 relative to respective untreated platelets, #P ≤ .05 between ACC WT and ACC DKI platelets. The data underwent 2-way ANOVA.