Lack of AMPK-ACC signaling results in an increased AA-containing PL pool. (A,B) The percentage (dark gray bar) of down- or upregulated lipid species relative to the whole lipid class was calculated in collagen-stimulated platelets relative to unstimulated platelets (A) or ACC DKI relative to ACC WT platelets (B). The different lipid classes are arranged from top down in order of increasing adjusted P values. (A) *Adjusted P ≤ .05 between collagen-stimulated platelets and unstimulated platelets (n = 3). (B) #P ≤ .05 between ACC DKI and WT platelets (n = 3). Multivariate regression analysis was performed. (C) Comparison of AA-containing PEP and PC between ACC DKI and WT platelets. The data are presented as log fold change (squares) relative to ACC WT platelets and with 95% confidence interval (CI) (horizontal lines). Statistical significance was based on false discovery rate P values (adjusted P values). The different lipid species are arranged from top down in order of increasing adjusted P values. Multivariate regression analysis was performed. #P ≤ .05 between ACC WT and ACC DKI platelets. (D) Proportions of PE(P-16:0/20:4), PE(P-18:1/20:4), PC(18:2/20:4), and PC(14:0/20:4) relative to all AA-containing PL were evaluated in ACC WT platelets. See also supplemental Figure 6 and 7 and supplemental Table 2 and 3. OR, odds ratio; n.a., not applicable (if no change or if change is OR < 1).