ASB2α regulates ECM degradation and migration through matrigel-coated filters. ASB2α^−/− and ASB2α^+/+ BMDCs were harvested and serum arrested for 1 hour in suspension. Cells were then plated on gelatin-coated coverslips and were fixed after 6 hours (A-B) or seeded onto matrigel-coated filters (C-D). (A) Images of ASB2α^+/+ and ASB2α^−/− BMDCs degrading FITC labeled gelatin coated on glass coverslips. Vinculin staining is shown in red and DAPI staining of the DNA in purple (B) Quantification of the area of FITC-labeled gelatin degraded by ASB2α^+/+ and ASB2α^−/− BMDCs. Dot plots show the overall distribution, and lines show the median values. (Sample size: ^+/+ = 5; ^−/− = 5). (C) Migration of ASB2α^+/+ and ASB2α^−/− BMDCs trough matrigel-coated transwells. The percentage of BMDCs that migrated to the bottom well is shown. (Sample size: ^+/+ = 7; ^−/− = 5). (D) Images of ASB2α^+/+ BMDCs that were seeded onto matrigel-coated filters (before migration) or that were recovered at the bottom wells (migrated cells). Cells were centrifuged onto poly-l-lysine–coated glass coverslips, fixed, and stained for FLNa (green) and 4,6 diamidino-2-phenylindole (DAPI; purple). Dot plots show the percentages of migrated FLNa-negative (FLNa⁻) and FLNa-positive (FLNa⁺) BMDCs obtained from ASB2α^+/+ mice. Scale bar represents 20 μm. (Sample size: 6). *P < .05; **P < .01.