OxLDL induced phosphorylation of MLC are MLCK, Ca²⁺, Rho kinase dependent. (A) Platelets (3 × 10⁸/mL) were treated with ML-7 (5 µM) for 20 minutes, followed by stimulation with oxLDL (50 µg/mL) for 15 seconds. Samples were then lysed, separated by SDS-PAGE, and immunoblotted for phospho-MLC^Ser19 followed by reprobing for β-tubulin. (Ai) Representative blots. (Aii) Densitometric analysis of 5 independent experiments. *P < .05. (B) Platelets (3 × 10⁸/mL) were treated with Y27632 (10 µM), BAPTA-AM (20 µM), or a combination of both or DMSO (0.1%) for 20 minutes followed by stimulation with oxLDL (50 µg/mL) for 15 seconds. Samples were then processed as in (A). (Bi) Representative blots. (Bii) Densitometric analysis of 4 independent experiments. All experiments were carried out in the presence of apyrase (2 U/mL), indomethacin (10 µM), and EGTA (1 mM). Data are expressed as mean ±SEM. *P < .05.