Srsf2P95H/+HSCs have a competitive advantage when transplanted with age- and microenvironment-matched support BM. (A) Experimental outline. (B-C) PB chimerism and repopulating units from 2 independent competitive transplantation experiments with BM from R26-CreER Srsf2+/+ (control) and R26-CreER Srsf2P95H/+ animals at 24 weeks after Cre activation (n = 3-5 recipients per transplantation cohort per experiment). (D-E) Fluorescence-activated cell sorting plots for PB CD11b/Gr-1 populations and lineage distribution at 4 weeks posttransplantation from R26-CreER model. (F) Experimental outline for hScl-CreER model transplantation. (G) PB leukocytes, mean cell volume (MCV), red blood cell counts, and hematocrit over 50 weeks posttransplantation. (H) PB chimerism as measured by CD45.2 staining. (I) eYFP levels within the CD45.2+ fraction for each genotype. (J) Percentage of eYFP+ granulocytes in the PB over time. Presented as mean ± standard error of the mean. Student t test or analysis of variance with repeated measures. *P < .05, **P < .01. BMT, BM transplantation; CRU, competitive repopulating unit.
