Jarid1b knockdown decreases hematopoietic cell differentiation in vitro. (A) Left panel: Jarid1b knockdown increases yields of mononuclear cells in cultures initiated with shJarid-transduced HSC/progenitor cell populations (mean ± standard deviation (SD), n = 2). Right panel: Wright-stained cytospin preparations of cells on day 9 of culture, 40× magnification, white arrows indicate primitive cells. (B) shJarid1b suppresses in vitro differentiation of HSC/progenitor cell populations. Proportions of Gr1⁺ cells on day 9 of culture were determined by flow cytometry. Each dot represents an independent culture. (C) Jarid1b knockdown enhances in vitro expansion of myeloid CFCs. The increase in CFC numbers was calculated from MNC and CFC numbers determined on days 2 and 9 (mean ± SD, n = 4). (D) Images of the predominant colony types. shJarid1b, colony-forming unit granulocyte-macrophage (CFU-GM), high proliferative potential; shMoz, CFU-macrophage (CFU-M), low proliferation. Left panels, bright field; right panels, epifluorescence. (E) Proportions of the highly proliferative CFU-GM in cultures of shRNA-transduced cells (mean ± SD, n = 4). (F) qRT-PCR–based comparison of cell fate–associated transcript levels on day 7 (Figure 3A) shJarid1b and shMoz cells compared with shLuc controls.