Development of mouse NK cells determined by CD27 and CD11b expression in multiple mouse organs. NK cells were identified as FSC-H vs FSC-A singlet and viable cells and then as CD45⁺NKp46⁺CD3⁻ cells. Quadrant gates were set using fluorescence minus 1 with isotype controls (for example, see supplemental Figure 2).Circles represent control Npc1^+/+ animals and squares represent affected Npc1^−/− animals; ages are ±1 day. Data are presented as mean ± SEM, n = 3 to 7 animals.*P < .05, **P < .01, and ***P < .001, calculated by an unpaired t test comparing to the age-matched controls, using GraphPad Prism v4.