Mortalin KD leads to a severe deficiency in long-term HSC repopulation. (A) The experimental scheme is shown. (B) Mortalin expression in sh-mortalin–transduced LSK cells at 10 days after transduction. Data represent the mean ± SD (n = 3; ***P < .001). (C) Numbers of CD150⁺ LSK cells 1 week after the culture of sorted sh-mortalin–transduced GFP⁺ LSK cells. Data represent the mean ± SD (n = 4; **P < .01). (D) PB chimerism of donor-derived cells analyzed at each month posttransplantation. Data represent the mean ± SD (n = 7/group; **P < .01). (E) Absolute number of donor-derived MNCs in the BM at 4 months posttransplantation. Data represent the mean ± SD (n = 7/group; **P < .01, ***P < .001). (F) Number of colony forming cells originating from 500 donor-derived LSK cells at 4 months posttransplantation. Data represent the mean number of colonies (±SD) formed per 500 cells (n = 3/group; **P < .01). (G) Percentage (±SD) of donor-derived B220⁺ B cells, CD3⁺ T cells, and Mac-1⁺/Gr-1⁺ myeloid cells in the recipient BM (n = 7/group). (H) Representative cell cycle FACS profiles (left) of GFP⁺ donor-derived LSK cells at 4 months posttransplantation. Graphs (right) show the mean percentages (±SD) of donor-derived LSK cells in the Go state (n = 7/group; **P < .01). (I) Percentages (±SD) of donor-derived Annexin V⁺ LSK cells in the recipient BM (n = 7/group).