American Society of Hematology

Figure 4

$Figure 4. BeWo cell monolayer transfer of wild-type IgG1 and engineered antibody. Antibody (α-D IgG1, IgG3ΔHinge, or IgG3ΔHinge:R435H) was added to the apical side of individual wells and medium fractions were collected from the apical and the basolateral reservoirs. The amount present in each fraction was measured by ELISA for quantitation of IgG and transfer was expressed as the basolateral:apical (B/A) ratio. Transfer of IgG3ΔHinge:R435H is significantly larger than transfer of IgG3ΔHinge.$

BeWo cell monolayer transfer of wild-type IgG1 and engineered antibody. Antibody (α-D IgG1, IgG3ΔHinge, or IgG3ΔHinge:R435H) was added to the apical side of individual wells and medium fractions were collected from the apical and the basolateral reservoirs. The amount present in each fraction was measured by ELISA for quantitation of IgG and transfer was expressed as the basolateral:apical (B/A) ratio. Transfer of IgG3ΔHinge:R435H is significantly larger than transfer of IgG3ΔHinge.

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