Dasatinib has no stimulatory effect on mouse DCs. (A) Immature DCs were generated from C57Bl/6.CD45.1 bone marrow cells and matured overnight with 100 ng/mL of LPS and 100 IU/mL of INFγ with or without titrated doses of dasatinib. After 16 hours, supernatant was collected and the IL-12p70 concentration was evaluated by ELISA (pooled data from 3 experiments). (B) Splenic T cells from C57Bl/6.pmel-1 mice, engineered to all express a TCR specific for the mgp100-peptide, were isolated and cocultured with gp100-loaded, differentially treated DCs for 96 hours. Then viable T cells were counted using an automated cell counter (data represent technical replicates, representative for 3 independent experiments).