Expanded CD11c^hiCD123^–CD14^– cells are fibrocytes that mediate angiogenesis. (A) Using the same gating strategy as shown in Figure 1A, CD11c^hiCD123^–CD14^– cells from a representative subject sample were analyzed for cell surface phenotype. The shaded areas represent background fluorescence on the designated population as indicated by FMO controls. This is representative of more than 10 experiments. (B) Comparison of tube formation by both electronically sorted CD11c^hiCD123^–CD14^– cells (designated fibrocytes) and CD14⁺ monocytes from 1 representative cancer subject, which were then plated in an angiogenesis assay. HUVEC cultures in FGF-2 were used as a positive control. This is representative of 5 experiments. (C) Mean ± SEM joint counts formed by HUVEC cocultured with FGF-2, monocytes vs fibrocytes in 5 separate tube formation assays using fibrocytes, and monocytes from 5 separate subjects.