Frequency of circulating fibrocytes in cancer subjects correlates with the expansion of circulating GATA3⁺CD4⁺ T cells. (A) Correlation between % fibrocytes in the monocyte-rich apheresis fraction and GATA3⁺CD4⁺ T cells in the lymphocyte-rich apheresis fraction acquired simultaneously. Each closed dot represents a value measured in an individual subject from whom adequate simultaneous monocyte-rich and lymphocyte-rich samples were available (n = 22 subjects). Gray dots represent results from normal donors (P < .05). (B) Representative flow cytometry dot plot illustrating high levels of GATA3⁺CD4⁺ T cells from 1 subject with simultaneous fibrocyte expansion. CD4⁺ cells were analyzed immediately after thawing and permeabilization without stimulation.