Loss of β2–kindlin-3 interactions in CD4 T cells results in altered homing in vivo. (A) Expression levels of the adhesion molecules PSGL-1, CD43, β1 integrin, CD44, and CD62L in WT (solid line) and KI (dashed line)-naïve CD4 T cells. (B) Naïve CD4 T cells were isolated from WT and KI mice, labeled with CFSE and CellTrace Violet, respectively, and mixed at a 1:1 ratio. (C) After adoptive transfer into recipient WT mice, the localization of WT and KI donor cells in the organs indicated was analyzed 16 hours post transfer. Plotted values indicate the recovery of WT or knock-in donor cells as a percentage of total recovered cells. Data are pooled from 2 independent experiments: 5 mice per group per experiment. Each data point represents an individual mouse, with the mean indicated. The dashed line shows 1:1 recovery of WT and KI T cells. Deviation from the dashed line indicates differential homing of WT and KI cells. The minimal, but significant, defect in KI T-cell homing to the Peyer’s patches likely reflects the involvement of other integrins in access to gut-associated lymphoid tissue. Student t test was used to calculate P values. NS, not significant.