Gene-expression analysis. (A) PCA of 8 sets of preinfusion (blue), infusion (green), and postinfusion (red) samples analyzed by NanoString. (B) Venn diagram showing the number of differentially expressed genes identified in each of the 3 comparisons, among a code set of 511 targets. (C) Volcano plot for the 511 genes, comparing matched postinfusion and infusion engineered T cells. Horizontal line at y = 1.31 represents the threshold of statistical significance (P = .05) and vertical lines at x = ^+/−2 represent the threshold of FC set as cutoff values for the definition of differentially expressed genes.