CD160 is a marker of T cells with reduced IFNγ-secretion capacity. (A) Representative example of IFNγ intracellular staining in 1-month postinfusion MAGE A3 TCR⁺ CD8 lymphocytes (gated separately based on CD160 expression) cocultured against MAGE A3⁺ 624 and H1299-A2, or HVEM-transduced derivatives. (B) Summary of results from 10 infusion samples and 8 postinfusion samples cocultured with 624 or 624-HVEM. (C) Intracellular staining of IFNγ in infusion and postinfusion lymphocytes of patient 9 stimulated with phorbol 12-myristate 13-acetate (PMA)/Ionomicyn during 4 hours or nonstimulated controls. (D) Summary of 6 patients.