CD30 expression in PEL cell lines and primary tumors. (A) Histogram of flow cytometric analysis shows CD30 staining on the indicated PEL cell lines and control WSU-NHL cell line. Data were obtained by acquiring 1 × 104 cells stained with the anti-CD30-fluorescein isothiocyanate (FITC) antibody. Blue line indicates CD30-stained cell population and black line indicates background fluorescence observed with isotype control. (B-D) CD30 expression in primary PEL cells. Malignant cells from PEL patient (case 1) are highlighted with Papanicolaou stain (I). Immunocytochemistry analysis shows positive staining for epithelial membrane antigen (EMA) (II), HHV-8 latent antigen LANA (nuclear stain) (III), and CD30 (IV). Original magnification: I, III, IV ×100 and II ×50. (C) Large and discohesive malignant cells from PEL patient (case 2) are highlighted with hematoxylin and eosin stain (I). Immunocytochemistry analysis shows positive staining for EMA (II), HHV-8 latent antigen LANA (nuclear stain) (III), and CD30 (IV). Original magnification: I-IV ×100. (D) Top shows hematoxylin and eosin staining of PEL patient (case 3) lymphoma cells and bottom shows an immunohistochemistry stain for CD30 in neoplastic cells of the same PEL patient. Original magnification ×600.
