American Society of Hematology

Figure 2

$Figure 2. SCL rescues apoptosis induced a by a dominant negative or an antisense Scl in Kit+ hematopoietic progenitors. (A) SCL-YFP and ΔbSCL-GFP or SCL-YFP and asSCL-GFP were codelivered in WT bone marrow cells for 2 days, and apoptosis was monitored 48 hours after infection. (B) Annexin-V positive cells are illustrated in red. Note that red dots are present in the YFP− fractions, exclusively. Numbers in red represent the % of Annexin-V+ cells within each quadrant. (C) SCL-YFP+ and SCL-YFP− fractions were further analyzed for Kit expression and Annexin-V labeling in ΔbSCL- or asSCL-expressing cells. (D) Apoptosis in Kit+ cells. Data shown are the mean ± SD of n independent experiments.$

SCL rescues apoptosis induced a by a dominant negative or an antisense Scl in Kit⁺hematopoietic progenitors. (A) SCL-YFP and ΔbSCL-GFP or SCL-YFP and asSCL-GFP were codelivered in WT bone marrow cells for 2 days, and apoptosis was monitored 48 hours after infection. (B) Annexin-V positive cells are illustrated in red. Note that red dots are present in the YFP⁻ fractions, exclusively. Numbers in red represent the % of Annexin-V⁺ cells within each quadrant. (C) SCL-YFP⁺ and SCL-YFP⁻ fractions were further analyzed for Kit expression and Annexin-V labeling in ΔbSCL- or asSCL-expressing cells. (D) Apoptosis in Kit⁺ cells. Data shown are the mean ± SD of n independent experiments.

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