Expansion of licensed but not unlicensed Ly49H⁺ NK cells with increased IFNγ production post-HSCT in MHC-disparate recipients following MCMV infection. (A) Example flow plots of CD3-NK1.1⁺ NK cells gated on H-2^d vs H-2^b MHC-I haplotypes from splenocytes of B10.D2 (H-2^d), B10 (H-2^b), and C57BL/6 (H-2^b) host mice 7 days post-MCMV infection and that underwent allo-HSCT 10 or 17 days prior to MCMV infection. (B-D) Splenocytes were stained for CD3, NK1.1, Ly49H, Ly49C/I, and Ly49G2 7 days after infection post-HSCT and compared with noninfected mice. Percentage of CD3⁻ NK1.1⁺ Ly49C/I⁺/H⁺ and Ly49G2⁺/H⁺ cells in (B) B10, (C) B10.D2, (D) C57BL/6 infected and noninfected host mice are illustrated. (E-G) The percentage of CD3⁻ NK1.1⁺ Ly49H⁺ IFNγ⁺ Ly49G2⁺ or Ly49C/I⁺ NK cells was determined from infected and noninfected mice after allo-HSCT in (E) B10, (F) B10.D2, and (G) C57BL/6 mice. Data are representative of 2 to 3 experiments with 4 mice per group. The error bars represent the standard error of the mean. Statistical analysis was performed using 1-way ANOVA and Tukey posttest.*P < .05; **P < .01.