sGARP induces RORγt and IL-17 expression in naïve CD4⁺ T cells. Naïve CD4⁺ T cells were stimulated with anti-CD3 and anti-CD28 mAb in the presence or absence of sGARP (1 µg/mL) and with and without a combination of IL-6 (200 IU/mL) and IL-23 (100 ng/mL). RORγt mRNA expression 4 hours, 16 hours, and 72 hours (A) and IL-17A mRNA expression 72 hours (B) after stimulation was analyzed and normalized to EF1α and untreated cells calculated by δ-δ cycle threshold method. Results are representative of 3 independent experiments (mean ± SEM, *P < .05, n = 3).