A single treatment of RO heparins (20 or 120 mg/kg) abolished LPS-dependent induction of hepcidin in mice. Mice (8-9 weeks old) were treated with LPS (1 g/kg) with or without a single dose of RO-heparins (20 or 120 mg/kg of RO-82 or RO-68) and euthanized after 6 hours. qRT-PCR was used for mRNA quantification of (A) hepcidin, (B) Id1, (C) Socs3, and (D) Crp in relationship to Hprt1 mRNA. The values are presented with box plot histograms and are expressed as fold increase (1) or as percentage (100%) of untreated mice. (E) Serum iron was measured spectrophotometrically.